Compositions and methods for hydroxylating epothilones

Details Compositions and methods for hydroxylating epothilones Compositions and methods for hydroxylating epothilones

2007-05-08

2007-05-08

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C536S023100, C536S023200, C435S189000, C435S471000, C435S320100, C435S252300

Reexamination Certificate

active

10915172

ABSTRACT:
Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

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