Food or edible material: processes – compositions – and products – Products per se – or processes of preparing or treating... – Poultry egg is basic ingredient
Reexamination Certificate
1998-09-29
2001-01-23
Weier, Anthony J. (Department: 1761)
Food or edible material: processes, compositions, and products
Products per se, or processes of preparing or treating...
Poultry egg is basic ingredient
C426S002000
Reexamination Certificate
active
06177121
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to an animal food composition effective in reducing the level of cholesterol in an egg and a method of producing low cholesterol eggs. More specifically, the invention relates to an animal food composition including an animal food and a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor. The invention further relates to a method of producing low cholesterol eggs by administering an HMG-CoA reductase inhibitor to an egg-laying animal. The eggs so produced are also described.
BACKGROUND OF THE INVENTION
Coronary heart disease is one of the leading causes of death in the United States. The relationship between elevated plasma cholesterol levels, especially plasma low density lipoprotein (LDL) cholesterol, and an increased risk of contracting coronary heart disease has been well established. In light of this relationship, a dietary cholesterol intake of less than 300 mg/day has been recommended for all Americans (National Institutes of Health Consensus Development Panel, 1985). Foodstuffs containing lowered levels of cholesterol are therefore desired.
Each chicken egg contains about 215 mg cholesterol. Efforts at reducing the level of cholesterol in intact chicken eggs have included genetic selection, use of low fat and high fiber diets, administration of pharmacological agents and various egg selection methods. Many of these methods are tedious and others have not led to large decreases in egg cholesterol. An animal food composition effective in reducing the amount of cholesterol in an egg and a method of producing low cholesterol eggs are therefore needed. The present invention addresses these needs.
SUMMARY OF THE INVENTION
The present invention relates to an animal food composition including an animal food and an HMG-CoA reductase inhibitor. The animal food composition is preferably an oviparous vertebrate food composition. The animal food composition is effective in reducing the amount of cholesterol in an egg. The HMG-CoA reductase inhibitor is preferably a compound having the following formula:
wherein
R
1
is C
3-10
cycloalkyl; C
1-10
alkyl provided that the C
1-10
alkyl is not 1-methylpropyl if R
2
and R
3
are both methyl groups; C
1-10
CF
3
-substituted alkyl; phenyl; halophenyl; phenyl-C
1-3
alkyl; substituted phenyl-C
1-3
alkyl in which the substituent is halo, C
1-3
alkyl or C
1-3
alkoxy;
R
2
is H, C
1-10
alkyl, or hydroxyl;
R
3
is H, C
1-10
alkyl;
the dotted lines at X, Y and Z represent possible double bonds, said double bonds when present being either X and Z in combination or X, Y or Z alone;
or the corresponding dihydroxy acid, a pharmaceutically acceptable salt of said acid, a C
1-4
alkyl ester of said acid, a phenyldimethylamino-substituted-C
1-4
alkyl ester of said acid or an acetylamino-substituted-C
1-4
alkyl ester of said acid.
In yet another embodiment of the present invention, the HMG-CoA reductase inhibitor is a compound of the following formula:
wherein
X is —CH
2
—, —CH
2
CH
2
—, —CH
2
CH
2
CH
2
— or —CH
2
CH(CH
3
)—;
R
1
is 1-naphthyl; 2-naphthyl; cyclohexyl; norbornenyl; 2-, 3-, or 4-pyridinyl; 2-, 3- or 4-pyridinyl-N-oxide; 2-, 3- or 4-(N—C
1-4
alkyl) pyridinium halide; phenyl; phenyl substituted with halogen, hydroxyl, trifluoromethyl, C
1-4
alkyl, C
1-4
alkoxy, C
2-8
alkanoyloxy; C
1-8
alkyl;
R
2
and R
3
are independently hydrogen; cyano; trifluoromethyl; phenyl; C
1-4
alkyl; C
2-8
carboalkoxy; CH
2
OR
7
where R
7
is hydrogen, C
1-6
alkanoyl, —CONHR
8
where R
8
is a C
1-6
alkyl, phenyl or phenyl substituted with halogen or C
1-4
alkyl; halogen provided that if R
1
is 1-methylethyl, R
4
is 4-fluorophenyl and either of R
2
or R
3
is bromine, the other of R
2
or R
3
is not bromine; or
either of R
2
or R
3
is —CONR
5
R
6
where R
5
and R
6
are independently hydrogen; C
1-6
alkyl; 2-, 3- or 4-pyridinyl; phenyl; phenyl substituted with halogen, cyano, trifluoromethyl or C
3-8
-carboalkoxy; and the other of R
2
or R
3
is hydrogen; C
1-6
alkyl; cyclopropyl; cyclobutyl; cyclopentyl; cyclohexyl; phenyl; phenyl substituted with halogen, hydroxyl, trifluoromethyl, C
1-4
alkyl, C
1-4
alkoxy, or C
2-8
alkanoyloxy; and
R
4
is C
1-6
alkyl, cyclopropyl; cyclobutyl; cyclopentyl; cyclohexyl; trifluoromethyl; phenyl; or phenyl substituted with halogen;
or the corresponding dihydroxy acid.
In yet another embodiment of the invention, a method of lowering the amount of cholesterol in an egg is provided that includes administering to an egg-laying animal an effective amount of the above-described compounds. The method is preferably carried out with chickens but may be carried out with any oviparous vertebrate, including turkey, geese, quail, rattites (including ostriches, emus and rheas) turtles and fish.
In a further embodiment of the present invention, an egg produced by the above-method is also described.
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Elkin Robert G.
Yan Zhihong
Purdue Research Foundation
Weier Anthony J.
Woodard Emhardt Naughton Moriarty & McNett
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