Combined use of .beta.-galactosidase and sialyltransferase coupl

Details Combined use of .beta.-galactosidase and sialyltransferase coupl Combined use of .beta.-galactosidase and sialyltransferase coupl

1993-05-04

1994-12-20

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 97, 435101, 435175, 435193, C12P 1904, C12P 1912, C12N 910

Patent

active

053745418

ABSTRACT:
A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a .beta.-galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an .alpha.-sialyltransferase. The value of K.sub.m /V.sub.max for the formed galactosyl glycoside as a substrate for the .alpha.-sialyltransferase is less than one-third the K.sub.m /V.sub.max value for the galactose-containing substrate for that .alpha.-sialyltransferase.

REFERENCES:
patent: 4918009 (1990-04-01), Nilsson
patent: 5180674 (1993-01-01), Roth
patent: 5246840 (1993-09-01), Nilsson
patent: 5278299 (1994-01-01), Wong et al.
Ichikawa et al., J. Am. Chem. Soc., 113(12):4698-4700 (1991).
David et al., Adv. Carbohyd. Chem. Biochem., 49:175-237 (1991).

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