Codon optimised recombinant Dermaphagoides allergens

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023200, C435S320100, C435S325000, C435S070100

Reexamination Certificate

active

10297563

ABSTRACT:
The present invention relates to codon optimised polynucleotides which are efficiently expressed in mammalian cells and encode insect proteins fromDermaphagoidsdust mite. In particular, the optimised codon polynucleotides encode a protein fromDermaphagoides pteronyssinus,such as DerP1 or proDerP1. The present invention also provides methods of preparing pharmaceutical compositions comprising the expression of the codon optimised polynucleotides, and vectors and transformed host cells comprising them.

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patent: 6455686 (2002-09-01), McCall et al.
patent: WO 97/31115 (1997-08-01), None
patent: WO 99/25823 (1999-05-01), None
Andre et al., “Increased Immune Response Elicited by DNA Vaccination with a Synthetic GP120 Sequence with Optimized Codon Usage”,Journal of Virology, 72(2): 1497-1503 (1998).
Yang et al., “Optimized Codon Usage and Chromophore Mutations Provide Enhanced Sensitivity with the Green Fluorescent Protein”,Nucleic Acids Research, 24(22): 4592-4593 (1996).
Kim et al., “Codon Optimization for High-Level Expression of Human Erythropoietin (EPO) in Mammalian Cells”,Gene: an International Journal on Genes and Genomes, 199(1-2): 293-301 (1997).
Kawamoto et al., “Cloning and Expression of Der f 6, A Serine Protease Allergen from the House Dust Mite, Dermatophagoides Farinae”,Biochimica et Biophysica Acta, 1454(2): 201-207 (1999).
Hale, et al.,Protein Expression and Purification, vol. 12 pp. 185-188 (1998).

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