Cloning vector plasmid, vector-primer derived therefrom and prep

Details Cloning vector plasmid, vector-primer derived therefrom and prep Cloning vector plasmid, vector-primer derived therefrom and prep

1996-09-09

1998-07-21

Degen, Nancy

Chemistry: molecular biology and microbiology

Vector, per se

536 231, 536 234, 536 241, C12N 1563, C12N 1566

Patent

active

057834423

ABSTRACT:
A cloning vector plasmid suitable for preparing cDNA banks is disclosed. The cloning vector plasmid of the present invention comprises, in the order mentioned from upstream to downstream unless otherwise specified, a promoter PR1 acting in mammalian cells; a unique restriction enzyme site RE1 rarely existing in eukaryotic DNA and a transcription promoter PR2; a unique restriction enzyme site RE2 generating a 3'-protruding dG tail; a unique arbitrary restriction enzyme site RE3; a unique arbitrary restriction enzyme site RE4 generating a 3'-protruding terminus; a unique restriction enzyme site RE5 rarely existing in eukaryotic DNA and a transcription promoter PR3; and at least one kind of arbitrary restriction enzyme site RE6 generating a 3'-protruding terminus existing at an arbitrary position before the restriction site RE1; and further, at arbitrary positions other than the above-described restriction enzyme sites and promoters, an origin OR1 for replication in mammalian cells, an origin OR2 for replication of a single-stranded phage, an origin OR3 for replication in E. coli, and a selective marker.

REFERENCES:
patent: 4843003 (1989-06-01), Henikoff et al.
patent: 5238834 (1993-08-01), Pruitt
patent: 5624826 (1997-04-01), Kato et al.
Pharmacia Catalog "Molecular Biologicals" (1986) 67-68.
Mead et al., (1986) Protein Engineering, 1, 67-74.
Deininger (1987) Meth. Enzy. 152, 371-389.
Sargent et al. (1983) Science 222, 135-139.
Okayama et al. (1983) Molec. Cell. Bio. 3, 280-289.
Palazzolo et al., (1987) Gene, vol. 52, pp. 197-206.
Pruitt, Gene, (1988) vol. 66, pp. 121-134.
Helfman et al. (1983) PNAS 80, 31-35.

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