Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-06-14
1997-12-02
Vogel, Nancy T.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4353201, 43525233, C12N 1567, C12N 1500, C12N 1570, C12N 121
Patent
active
056934890
ABSTRACT:
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
REFERENCES:
Steen et al. (1986) EMBO J. vol. 5(5) pp. 1099-1105.
Tabor et al. (1985) PNAS vol. 82 pp. 1074-1078.
Mizusawa et al. (1982) Gene vol. 20 pp. 317-322.
Davauloo et al. (1984) PNAS vol. 81 pp. 2035-2039.
Mofflett et al., J. Mol. Biol., 173:265-269 (1984).
Studier et al., Dunn et al., J. Mol. Biol., 166: 477-535 (1983).
Studier et al., Cold Spring Harbor Symp. Quant. Biol., 47:999-1007 (1983).
Davanloo Parichehre
Dunn John J.
Moffatt Barbara A.
Rosenberg Alan H.
Studier F. William
Associated Universities Inc.
Bogosian Margaret C.
Vogel Nancy T.
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