Cloning and expression of the exo-polygalacturonase gene from as

Details Cloning and expression of the exo-polygalacturonase gene from as Cloning and expression of the exo-polygalacturonase gene from as

1997-01-24

1998-11-03

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 4352543, 4353201, 536 232, C12N 926, C12N 1556

Patent

active

058307370

ABSTRACT:
The exo-polygalacturonase gene from Aspergillus is identified, and the enzyme encoded thereby is expressed. Specifically, the exo-polygalacturonase gene from Aspergillus tubingensis is is cloned and expressed, and DNA sequences from other Aspergillus strains that specifically hybridize therewith are identified. Novel expression vectors that comprise an exo-polygalacturonase encoding sequence, and host cells transformed therewith, are also provided. The invention further relates to the production of recombinant exo-polygalacturonase, and to the use of this protein.

REFERENCES:
Bussink, H. J. D. et al., Curr. Genet., 19:467-474 (1991).
Hara et al., "Purification and Som Properties of Exo-polygalacturonase from Aspergillus niger Cultured in the Medium Containing Satsuma Mandarin Peel", Nippon Shokuhin Kogyo Gakkaishi 31(9):581-586 (1984).
He et al., "Molecular Cloning, Nucleotide Sequence, and Marker Exchange Mutagenesis of the Exo-Poly-.alpha.-D-Galacturonosidase-Encoding pehX Gene of Erwina chrysanthemi EC16", Journal of Bacteriology 172(9):4988-4995 (1990).
Kester et al., "Purification and Characterization of Polygalacturonases" Biotechnology and Applied Biochemistry 12:150-160 (1990).
Mill, "The Pactic Enzymes of Aspergillus niger", Biochem. J. 99:557-561 (1996).
Norz, "Wissenswertes uber Apfelpektin (Teil I)" ZSW May 1985 255-265 (1985).
Norz, "Wissenswertes uber Apfelpektin (Teil II)" ZSW Jun. 1985 303-306 (1985).
Pilnik et al., "The Biochemistry of Fruits and their Products", Academic Press (Hulme), vol. 1, 53-87 (1970).

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