Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-09-17
2000-04-25
Prouty, Rebecca E.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435232, 4353201, 4352523, 424945, C12N 1560, C12N 988
Patent
active
060545690
ABSTRACT:
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000.+-.5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000.+-.2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.
REFERENCES:
patent: 4390628 (1983-06-01), Johansen
patent: 4696816 (1987-09-01), Brown
patent: 5169772 (1992-12-01), Zimmerman et al.
patent: 5292509 (1994-03-01), Hageman
Baird and Klagsbrun, "The Fibroblast Growth Factor Family", Annals New York Academy of Sciences Nomenclature Meeting Report And Recommendations xi-xvi (1991).
Baker et al., "A Gene Regulating The Heat Shock Response In Escherichia Coli Also Affects Proteolysis", Proc. Natl. Acad. Sci., 81:6779-6783 (1984).
Cohen, et al., "Nonchromosomal Antibiotic Resistance In Bacteria; Genetic Transformation Of Escherichia Coli By R-Factor DNA", Proc. Natl. Acad. Sci. USA, 69:2110-2114 (1972).
Edman, "Phenylthiohydantoins In Protein Analysis", Ann. N.Y. Acad. Sci., 88:602-610 (1950).
Folkman, et al., "A Heparin-Binding Angiogenic Protein--Basic Fibroblast Growth Factor--Is Stored Within Basement Membrand", Am. J. Pathol., 130:393-400 (1988).
Folkman and Klagsbrun, "Angiongenic Factors", Science, 235:442-447 (1987).
Forrester, et al., "A Paradigm For Restenosis Based On Cell Biology; Clues For The Development Of New Preventive Therapies", J. Am. Coll. Cardiol., 17:758-769 (1991).
Galliher, et al., "Heparinase Production By Flavobacterium Heparinum", Appl. Environ. Microbiol., 41(2):360-365 (1981).
Gu, et al., "Comparison Of The Activity Of Two Chondroitin AC Lyases On Dermatan Sulfate", Carbohydrate Res., 244:369-377 (1993).
Higashiyama, et al., "A Heparin-Binding Growth Factor Secreted By Macrophage-Like Cells That Is Related To EGF," Science 251:936-939 (1991).
Laemmli, "Cleavage Of Structural Proteins During The Assembly Of The Head Of Bacteriophage T4", Nature, 227:680-685 (1970).
Linhardt, et al., "Polysaccharide Lyases", Appl. Biochem. Biotechnol., 12:135-177 (1986).
Linn, et al., "Isolation And Characterization Of Two Chondroitin Lysases From Bacteriodes Thetaiotaomicron", J. Bacteriol. 165:859-866 (1985).
Maniatis, et al., Molecular Cloning, A laboratory Manual (1982).
Michelacci, et al., "Isolation And Characterization Of An Induced Chondroitinase ABC From Flavobacterium Heparinum", Biochimica et Biophysica Acta, 923:291-301 (1987).
Miller, Experiments in Molecular Genetics, Cold Spring Harbor, 1972.
Nathan and Sporn, "Cytokines in Context", J. of Cell Biol., 113:981-986 (1991).
Richardson and Hatton, "Transient Morphological And Biochemical Alterations Of Arterial Proteoglycan During Early Wound Healing", Exp. Mol. Pathol., 58:77-95 (1993).
Sasisekharan, et al., "Heparinase Inhibits Neovascularization", Proc. Natl. Acad. Sci. USA 91:1524-1528 (1994).
Silhavy et al., in Experiments With Gene Fusions, Cold Spring Harbor Laboratory, 1984.
Southern, "Detection Of Specific Sequences Among DNA Fragments Separated By Gel Electrophoresis", J. Mol. Biol., 98:503-517 (1975).
Tabas, et al., "Lipoprotein Lipase And Sphingomyelinase Synergistically Enhance The Association Of Atherogenic Lipoproteins With Smooth Muscle Cells And Extracellular Matrix", J. Biol. Chem., 268(27):20419-20432 (1993).
Takegawa, et al., "Purification And Properties Of Chondroitinase Produced By A Bacterium Isolated From Soil", J. Ferm. Bioeng., 77 (2):128-131 (1991).
Vlodavsky, et al., "Endothelial Cell-Derived Basic Fibroblast Growth Factor: Snythesis And Deposition Into Subendothelial Extracellular Matrix", Proc. Natl. Acad. Sci. USA, 84:2292-2296 (1987).
Voss et al., "Automated DNA Sequencing System Resolving 1,000 Bases With Fluorescein-15-*dATP As Interanl Label", Meth. Molec. Cell. Biol., 3:153-155 (1992).
Werner, et al., "Large Induction Of Keratinocyte Growth Factor Expression In The Dermis During Wound Healing", Proc. Natl. Acad. Sci. USA, 89:6896-6900 (1992).
Yamagata, et al., "Purification And Properties Of Bacterial Chaondroitinases And Chondrosulfatases", The Journal Bio. Chemistry, 243(7):1523-1535 (1968).
Yang, et al., "Purification And Characterization Of Heparinase from Flavobacterium Heparinum", J. Biol. Chem., 160(30):1849-1857 (1985).
Yeo, et al., "Alterations In Proteoglycan Synthesis Common To Healing Wounds And Tumors", Am. J. Phatol., 138:1437-1450 (1991).
Dewent WPI Abstract No. 79-72510B, "Pure chondroitinase B Production", 1979.
Y. Zensaku et al., "Purification of Chomdroitinase B" Chem. Abstracts 92: 458, Abst. No. 92: 4710, Jan. 1980.
Y.M. Michelacci et al., "A Comparative Study Between a Chondroitinase B and a Chondroitinase AC From Flavobacterium heparinum" Biochem. J. 151: 121-129, Oct. 1975.
Bennett D. Clark
Fink Dominique
Gu Kangfu
Laliberte Maryse
Linhardt Robert
Ibex Technologies R and D, Inc.
Prouty Rebecca E.
LandOfFree
Chondroitin lyase enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chondroitin lyase enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chondroitin lyase enzymes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-994324