Cellulase tolerant to surfactants

Details Cellulase tolerant to surfactants Cellulase tolerant to surfactants

2008-05-20

2008-05-20

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S018000, C435S069100, C435S320100, C435S325000, C510S320000, C536S023200, C530S350000

Reexamination Certificate

active

07374921

ABSTRACT:
Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.

REFERENCES:
patent: WO 98/12307 (1998-03-01), None
patent: WO 01/90375 (2001-11-01), None
Shiarai, et al., “Crystal Structure of Alkaline Cellulase K: Insight Into The Alkaline Adaptation of an Industrial Enzyme”, Journal of Molecular Biology, London, GB, vol. 310, No. 5, Jul. 27, 2001, pp. 1079-1087.

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