Chemistry: molecular biology and microbiology – Spore forming or isolating process
4352402, 43524025, 43524026, 4352403, 435244, C12N 500, C12N 502, C12N 138
Cells grown in chemically defined (serum free) media are protected against endogenous oxidizing agents by a nontoxic antioxidant with free thiol groups, at cell densities less than about 10.sup.5 cells/ml, especially DL-penicillamine, N-acetylcysteine, mercaptoproprionic acid, 2-mercaptoethanesulfonic acid, and thiolactate. These agensts are useful in the maintenance and growth of hybridomas in such media.
Darfler et al., J. Cell Phys., 115:31-36 (1983), "Clonal Growth of Lymphoid Cells in Serum-Free Media Requires Elimination of H.sub.2 O.sub.2 Toxicity".
Broome et al., J. Exp. Med., 138: 574-592 (1973), "Promotion of Replication in Lymphoid Cells by Specific Thiols and Disulfides in Vitro".
Cell Enterprises, Inc.
Cooper Iver P.
Warren Charles F.
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