Cell-based assays employing voltage and calcium dyes

Details Cell-based assays employing voltage and calcium dyes Cell-based assays employing voltage and calcium dyes

2007-04-12

2009-10-20

Ulm, John D (Department: 1649)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S007100, C435S007200, C435S252300, C435S320100, C536S023500

Reexamination Certificate

active

07604959

ABSTRACT:
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

REFERENCES:
patent: 7166463 (2007-01-01), Karpen et al.
patent: 7341836 (2008-03-01), Karpen et al.
patent: 2003/0157571 (2003-08-01), Karpen et al.
patent: 03038039 (2003-05-01), None
Zong et al. Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channels. 1998,The EMBO Journal 17(2):353-362.
Liu et al. Clacium-calmodulin modulation of the olfactory cyclic nucleotide-gated cation channel, Nov. 25, 1994, Science 266:1348-1354.
Rich et al. In vivo assessment of local phosphodiesterase activity using taylored cyclic nucleotode-gated channels as cAMP sensors, Jul. 2001, Journal of General Physiology 118:63-77.
Rich et al. Cyclic nucleotide-gated channels colocalize with adenylate cyclase in regions of restricted cAMP diffusion. Jul. 17, 2000, Journal of General Physiology 116:147-161.
Gavazzo et al., “A point mutation in the pore region alters gatings CA2+ blockage, and permeation of olfactory cyclic nucleotide-gated channels”; Journal of General Physiology, 116(3) pp. 311-325 (Sep. 2000).

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