Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-04-19
2009-02-17
Chunduru, Suryaprabha (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024330
Reexamination Certificate
active
07491493
ABSTRACT:
Novel PCR based assays to detect Orthopoxvirus DNA have been developed which when tested under simulated clinical conditions have proven to be very sensitive, rapid and robust. Furthermore, artificial templates have been constructed which can be used as positive controls in the RFLP analysis necessary to differentiate variola and monkeypox viruses from the Orthopoxvirus species that normally cause relatively insignificant disease in man. These assays will provide a lab that has already undertaken the isolation of DNA from a patient sample in biocontainment with a sensitive and controlled assay for the detection and differentiation of human-tropic orthopox viruses.
REFERENCES:
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Loparev V N et al “Detection and Differentiation of old world orthopoxviruses: restriction fragment length polymorphism of the crmB gene region” Journal of Clinical Microbiology Jan. 2001, vol. 39, No. 1, Jan. 2001 pp. 94-100.
Ropp S L et al “PCR Strategy for identification and differentiation of small pox and other orthopoxviruses” Journal of Clinical Microbiology, Washington DC vol. 33 No. 8 Aug. 1995 pp. 2069-2076.
Meyer H. et al “Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses” Journal of Virological Methods, Amsterdam, vol. 64, No. 2, Mar. 1997 pp. 217-221.
Database Biosis “online” Biosciences Information Service, Philadelphia PA US 2003, Nie Z et al Detection and differentiation of variola virus from other orthopoxviruses using RFLP of the complement regulatory protein gene.
Czub Markus
Grolla Allen
Ade & Company Inc.
Chunduru Suryaprabha
Williams Michael R.
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