Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1998-12-15
Rories, Charles C. P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 691, 435 911, 435 914, 435 915, 435 9151, 4351723, 4353201, 536 231, 536 232, 536 241, 536 245, 536 251, 536 253, C12N 1563, C12N 1570, C12N 1579, C12N 1510
Patent
active
058495487
ABSTRACT:
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.
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Brand Andrea
Goodman Howard M.
Haseloff James
Perrimon Norbert
Rories Charles C. P.
The General Hospital Corporation
The President and Fellows of Harvard College
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