Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-08-01
2006-08-01
Prouty, Rebecca (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S254200, C435S069100, C435S320100, C536S023200
Reexamination Certificate
active
07083962
ABSTRACT:
An objective of the present invention is to provide methods for efficiently producing (S)-1-(3,4-dimethoxyphenyl)-2-propanol at a high optical purity. Another objective is to provide novel reductases which reduce 3,4-dimethoxyphenylacetone, using NADPH as a coenzyme, to produce (S)-1-(3,4-dimethoxyphenyl)-2-propanol with a high optical purity.The inventors found that a 3,4-dimethoxyphenylacetone-reducing enzyme present inTorulaspora delbrueckiiis a novel carbonyl reductase that reduces various carbonyls. This novel enzyme reduces 3,4-dimethoxyphenylacetone in a reduction reaction to produce (S)-1-(3,4-dimethoxyphenyl)-2-propanol with a high optical purity and at a high yield. Furthermore, the inventors isolated a DNA that encodes the present enzyme, and generated a recombinant bacterium which highly expresses the present enzyme. Thus, the present inventors established a simple and highly economical method of obtaining optically active alcohols with a high optical purity and at a high yield.
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Kimoto Norihiro
Nakajima Takanori
Yamamoto Hiroaki
Daicel Chemical Industries Ltd.
DiGiorgio, Esq. Jeanne M.
Gebreyesus Kagnew
Hanley, Esq. Elizabeth A.
Lahive & Cockfield LLP
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