Capture method for cell nucei using a DNA mesh

Details Capture method for cell nucei using a DNA mesh Capture method for cell nucei using a DNA mesh

1993-09-14

1995-09-05

Parr, Margaret

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435 2, 435174, 435177, 435178, 435179, 435180, 435181, 435182, 4352401, 435243, 435259, 435267, 4353171, 435803, 435820, 436 63, 436176, 436177, 436178, 536 221, 935 19, C12N 106

Patent

active

054478642

ABSTRACT:
A method of separating cell nuclei from cells comprises: treating a fluid containing whole cells so as to selectively lyse the cytoplasmic membrane together with a small proportion of the nuclear membranes but leaving a large proportion of the cell nuclei intact; applying the treated fluid to a membrane whereby a mesh of DNA from the lysed nuclei is formed on the surface and captures intact cell nuclei. A device for use in the method is described.

REFERENCES:
patent: 4668618 (1987-05-01), Thornthwaite
Leadon et al., Analytical Biochemistry, 120 (2), 282-288; (1982).

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