Capillary electrophoresis enzyme immunoassay

Details Capillary electrophoresis enzyme immunoassay Capillary electrophoresis enzyme immunoassay

1997-01-22

1999-09-28

Warden, Robert

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204601, 435 6, 435 77, 435 792, 436516, G01N 2726, G01N 3353, G01N 33561, C12Q 168

Patent

active

059582022

ABSTRACT:
Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.

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