Buffered wash composition, insolubilizing composition, test kits

Details Buffered wash composition, insolubilizing composition, test kits Buffered wash composition, insolubilizing composition, test kits

1989-12-07

1993-01-05

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 794, 435 28, 435971, 436510, 436518, 436532, 436814, G01N 3353, G01N 3376

Patent

active

051769999

ABSTRACT:
A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.

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