Buffered embryo solutions

Details Buffered embryo solutions Buffered embryo solutions

1995-10-05

1998-02-10

Ketter, James

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

C12N 502

Patent

active

057168474

ABSTRACT:
Aqueous buffered physiological solutions suitable for use with embryos are disclosed based on a selection of zwitterionic buffers, allowing improved embryo solutions to be produced, giving a greater holding time than conventional phosphate buffers. An embryo flushing solution and an embryo holding solution based on these bufers are described. A preferred buffered embryo holding solution contains the following ingredients: NACl, MOPS MgCl.sub.2.6H.sub.2 O, NaHCO.sub.3, Kanamycin Sulphate, Glucose, Bovine Albumin, Na Lactate, NaOH, and tissue culture grade water.

REFERENCES:
Miyoshi et al. Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity. J. Reproduction and Fertility vol. 100 pp. 21-26, 1994.
Pope et al. In vitro development of mouse wmbryos after cryopreservation in phosphate or Hepes buffered media in two different size straws. Theriogenology vol. 41 1613-1619, 1994.
Sigma Cell Culture 1994 Catalogue pp. 15, 28, 244, and 259, 1994.
Carnevale et al. Comparison of Ham's F10 with CO2 or Hepes buffer for storage of equine embryos at 5C for 24 H. J. Animal Science vol. 65 1775-1781, 1987.

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