Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2011-08-23
2011-08-23
Dunston, Jennifer (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S173500, C435S173600
Reexamination Certificate
active
08003389
ABSTRACT:
A method for introducing biologically active molecules into animal or human cells using electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution which has a buffer capacity of at least 20 mmol×l−1×pH−1and an ionic strength of at least 200 mmol×l−1at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. to form a suspension. The method further includes applying an electric voltage to the suspension so as to introduce the biologically active molecules into animal or human cells.
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Brosterhus Helmut
Helfrich Juliana
Lorbach Elke
Mueller-Hartmann Herbert
Nix Michael
Dunston Jennifer
Leydig , Voit & Mayer, Ltd.
Lonza Cologne GmbH
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