Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-01-16
1993-02-23
Kipplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 435174, 435189, G01N 33569, C12N 1553
Patent
active
051889366
ABSTRACT:
Diagnostic reagents comprising the 20 kd Brucella abortus CuZn superoxide dismutase (B. abortus lCuZnSOD) protein and peptide segments thereof, which are effective as antigenic determinants, have been identified. These reagents are useful for detecting an antibody response to the B. abortus CuZnSOD protein in bovine serum or other body fluid samples and can also be used for distinguishing between animals which have serum antibody of a natural B. abortus infection and those which have an antibody response to a B. abortus Strain 19 vaccine or a B. abortus Strain which does not express the 20 kd protein.
REFERENCES:
Tabatabai et al. "Specific Enzyme-Linked Immunosorbent Assay for Detection of Bovine Antibody to Brucella abortus", J. Clin. Microbiol. 20(2): 209-213 (Aug. 1984).
Hopp et al, "Prediction of protein antigenic determinants from amino acid sequences" Proc. Natl. Acad. Sci. USA 78(6): 3824-3828 (Jun. 1981).
Louisa B. Tabatabai et al., "Immunogenicity of Brucella abortus Salt-Extractable Proteins," Vet. Microbiol. 20: 49-58 (1989).
Bonnie L. Beck et al., "A Protein Isolated from Brucella abortus is a Cu-Zn Superoxide Dismutase," Biochemistry 29: 374-376 (1990).
Betsy J. Bricker et al., "Cloning, Expression, and Occurrence of the Brucella Cu-Zn Superoxide Dismutase," Infect. Immun. 58(9): 2935-2939 (Sep. 1990).
L. B. Tabatabai et al., "Characterization of Salt-Extractable Protein Antigens from Brucella abortus . . . " Vet. Microbiol. 9: 549-560 (1984).
M. Thompson et al., "Comparison of Brucella abortus Protein-Enzyme-Immunoassays and Conventional Serologic Tests for Detecting Bovine Antibody to B. abortus, "Abstr. #84, Iowa Academy of Science, Apr. 20-21, 1990.
Louisa B. Tabatabai et al., "Isolation and Characterization of Toxic Fractions from Brucella abortus," Infect. Immun. 26(2): 668-679 (Nov. 1979).
Beck Bonnie L.
Mayfield John E.
Tabatabi Louisa B.
Bidwell Carol E.
Fado John D.
Kipplinger Esther L.
Ribando Curtis P.
Silverstein M. Howard
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