Biosynthesis of isopentenyl pyrophosphate

Details Biosynthesis of isopentenyl pyrophosphate Biosynthesis of isopentenyl pyrophosphate

2007-02-06

2007-02-06

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S183000, C435S189000, C435S194000, C435S232000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

10006909

ABSTRACT:
Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

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National Science Foundation Award Abstract No. 9911463, Feb. 10, 2006.

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