Biomolecule immobilisation using atmospheric plasma technology

Coating processes – Direct application of electrical – magnetic – wave – or... – Plasma

Reexamination Certificate

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Details

C427S576000, C427S578000, C435S004000, C435S007100, C118S715000, C118S7230AN, C118S7230ER

Reexamination Certificate

active

07666478

ABSTRACT:
The present invention is related to a method for immobilising a biomolecule on a surface by generating and maintaining an atmospheric pressure plasma, the method comprising the steps of: introducing a sample in the space between two electrodes, a mixed atmosphere being present between the electrodes, applying an alternating voltage to the electrodes for generating and maintaining a plasma in the volumetric space between the electrodes, characterized in that the mixed atmosphere comprises an inert gas or nitrogen, an aerosol comprising a reactive precursor and an aerosol comprising a biomolecule, the reactive precursor and biomolecule being deposited and immobilized during the depositing step.

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Paulussen et al. “Antimicrobial coatings obtained in an atmospheric pressure dielectric barrier glow discharge” Mrs Symposium Proceedings Series vol. 724 Apr. 1, 2002 X-002347059.
Muguruma et al. “Plasma-polymerized films for biosensors” Trends in analytical Chemistry vol. 18, No. 1 p. 62-68 (1999).

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