Biological sensors

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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Details

422 8211, 422 66, 422 681, 356318, 356445, G01N 2100, G01N 2155, G01J 330

Patent

active

050230530

ABSTRACT:
A surface plasmon resonance (SPR) sensor in which a membrane is used to enable the sensitive material to be readily changed to enable tests to be carried out on a sequential basis simply by moving or changing the membrane. In one embodiment, the membrane takes the form of a continuous film (24) which lies against and is moveable across by means not shown, a transparent plate (17) which forms part of the SPR sensor. The film (24) comprises a layer (31) of flexible transparent material to which is applied a thin layer (32) of metal such as silver. Applied to layer (32) is a layer (33) of sensitive material. The final layer (34) is a preformed layer of hydrophyllic plastics material which incorporates troughs (37) and passageways (35,38) for the transfer of sample across the sensitive layer. Light from a laser (not shown) is subject to total internal reflection at the interface between the transparent layer (31) and metal layer (32), and the reflected light is collected by a light detector (not shown). Provided conditions are correct, surface plasmon resonance occurs at the area of total internal reflection, the characteristics of which resonance are sensitively dependent upon the reaction between the sample and the sensitive layer.

REFERENCES:
patent: Re30627 (1981-05-01), Bagshawe et al.
patent: 3526480 (1970-09-01), Findl et al.
patent: 4844613 (1989-07-01), Batchelder et al.
patent: 4857273 (1989-08-01), Stewart
patent: 4877747 (1989-10-01), Stewart
patent: 4883642 (1989-11-01), Bisconte
patent: 4889427 (1989-12-01), Van Veen et al.

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