Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1997-05-29
2000-05-02
Huff, Sheela
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 8201, 422 88, 422 98, 436149, G01N 2700
Patent
active
060569220
ABSTRACT:
An alkanethiol monolayer membrane is formed on a substrate having an Au layer using Au--S bonding, and a second layer constructed by a monolayer lipid membrane is formed on its surface to form a BLM. A gel layer is formed using agarose on the side of the BLM having the second monolayer lipid layer. In addition, a polymer layer is formed using the amino acid poly-L-lysine between the surface of the monolayer lipid membrane and the gel layer to immobilize the BLM. The use of this supporting constitution for holding and immobilizing the membrane extends the life-span of the BLM, while the gel layer keeps the electrolyte solution required for maintaining flexibility of the membrane and activating protein on the surface of the BLM. In addition, a BLM having a life-span of one month or more is obtained by arranging a membrane supporting portion that minimizes lifting of the membrane molecules due to buoyancy above the BLM arranged upright in an aqueous system.
REFERENCES:
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Karsten Seifert, et al., "Charge transport by ion translocating membrane proteins on solid supported membranes", Biophysical Society, vol. 64, Feb. 1993, pp. 384-391.
Claus Duschl, "Biologically Addressable Monolayer Structures Formed by Templates of Sulfur-Bearing Molecules", Biophysical Society, vol. 67, Sep. 1994, pp. 1229-1237.
Martin Stelzle, et al., "Sensitive detection of protein adsorption to supported lipid bilayers by frequency-dependent capacitance measurements and microelectrophoresis", 1989 Elsevier Science Publishers B.V., pp. 135-142.
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Huff Sheela
Sanyo Electric Co. Ltd
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