Bi-directionally cloned random cDNA expression vector...

Details Bi-directionally cloned random cDNA expression vector... Bi-directionally cloned random cDNA expression vector...

2007-04-17

2007-04-17

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091510, C435S091520, C435S320100, C435S325000, C435S489000, C536S023100

Reexamination Certificate

active

10142648

ABSTRACT:
The present invention provides random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in sense and antisense orientation, which are useful for the delivery and expression of a combination of genetic effector types to host cells. Methods for producing these libraries through bi-directional cloning of random cDNAs are also provided. Also provided herein are methods of using these libraries to screen for agents capable of modulating cell phenotype in desirable ways.

REFERENCES:
patent: 5595895 (1997-01-01), Miki et al.
patent: 6140086 (2000-10-01), Fox et al.
patent: 6544741 (2003-04-01), Mugasimangalam
patent: 6808906 (2004-10-01), Shen et al.
Gossen, et al. “Tight control of gene expression in mammalian cells by tetracycline-responsive promoters”,Proc. Nat. Acad. Sci. USA, (1992) vol. 89: 5547-5551.
Hofmann, et al. “Rapid retroviral delivery of tetracycline-inducible genes in a single autoregulatory cassette”,Proc. Natl. Acad. Sci. USA, (1996) vol. 93: 5185-5190.
Lorens, et al. “Retroviral delivery of peptide modulators of cellular functions”,Molecular Therapy, (2000) vol. 1(5): 438-447.
Matz, et al. “Fluorescent proteins from nonbioluminescent Anthozoa species”,Nature Biotechnology, (1999) vol. 17: 969-973.
Xu, et al. “Dominant effector genetics in mammalian cells”,Nature Genetics, (2001) vol. 27: 23-29.

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