Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1994-04-14
1997-04-29
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4352523, 43525411, 4353201, 435325, 435348, 435358, 435361, 536 232, C12N 510, C12N 910, C12N 1554, C12N 1563
Patent
active
056248326
ABSTRACT:
The present invention provides a novel .beta.1.fwdarw.6 N-acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in O-glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 .beta.1.fwdarw.6 N-acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed.
REFERENCES:
patent: 5179003 (1993-01-01), Wolf et al.
patent: 5320943 (1994-06-01), Fukuda
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Bierhuizen Marti F. A.
Fukuda Minoru
Grimes Eric
La Jolla Cancer Research Foundation
Wax Robert A.
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