Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1994-05-20
1996-03-26
Housel, James C.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
436 73, 436 79, 436172, 436 63, 548159, 548215, 548463, G01N 3320
Patent
active
055019803
ABSTRACT:
The indicator compounds of the present invention are substituted or unsubstituted BAPTA-type chelators that contain a benzazolyl-coumarin substructure, and the pharmaceutically acceptable non-toxic salts and esters thereof. These compounds are useful for the detection and quantification of polycationic metal ions, particularly Ca.sup.2+.
The compounds of the invention have the core structure ##STR1## or the structure ##STR2## where m=2 or 3 and X can be S, O, or C(CH.sub.3).sub.2. The above core structures are optionally substituted by substituents that alter the binding affinity of the indicator, shift the spectral properties of the indicator, or act as a reactive site for the preparation of a variety of conjugates.
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Foukaraki Evangelia
Haugland Richard P.
Iatridou Helene
Katerinopoulos Haralambos E.
Kuhn Michael A.
Helfenstein Allegra J.
Housel James C.
Molecular Probes, Inc.
Skaugset Anton E.
Soderquist Arlen
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