Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-05-05
1998-06-16
Elliott, George C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 435 9131, 4351721, C07H 2102, C12N 1500, C12P 1934
Patent
active
057672636
ABSTRACT:
Method to produce a more active ribozyme by introducing a modified base into a substrate binding arm of the ribozyme or its catalytic core.
REFERENCES:
patent: 4987071 (1991-01-01), Cech et al.
patent: 5298612 (1994-03-01), Jennings et al.
Adams et al., "A Convenient Synthesis of S-Cyanoethyl-Protected 4-Thiouridine and its Incorporation into Oligoribonucleotides," Tetrahedron Letters 35:765-768 (1994).
Bevilacqua and Turner, "Comparison of Binding of Mixed Ribose-Deoxyribose Analogues of CUCU to a Ribozyme and to GGAGAA by Equilibrium Dialysis: Evidence for Ribozyme Specific Interactions with 2' OH Groups," Biochemistry 30:10632-10690 (1991).
Chowrira and Burke, "Binding and Cleavage of Nucleic Acids by the `Hairpin` Ribozyme," Biochemistry 30:8518-8522 (1991).
Collins and Olive, "Reaction Conditions and Kinetics of Self-Cleavage of a Ribozyme Derived From Neurospora VS RNA," Biochemistry 32:2795-2799 (1993).
Fu et al., "Importance of Specific Guanosine N.sup.7 -Nitrogens and Purine Amino Groups for Efficient Cleavage by a Hammerhead Riobzyme," Biochemistry 32:10629-10637 (1993).
Fu and McLaughlin, "Importance of Specific Adenosine N.sup.7 -Nitrogens for Efficient Cleavage by a Hammerhead Ribozyme. A Model for Magnesium Binding," Biochemistry 31:10941-10949 (1992).
Fu and McLaughlin, "Importance of Specific Purine A,ino and Hydroxyl Groups For Effiicient Cleavage b a Hammerhead Ribozyme," Proc. Natl. Acad. Sci. USA 89:3985-3989 (1992).
Grasby et al., "The synthesis of oligoribonucleotides containing O.sup.6 -methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage," Nucleic Acids Research 21:4444-4450 (1993).
Guerrier-Takada et al., "The RNA Moiety of Ribonuclease P Is the Catalytic Subunit of the Enzyme," Cell 35:849-857 (1983).
Hampel et al., "`Hairpin` Catalytic RNA Model: Evidence for Helices and Sequence Requirement for Substrate RNA," Nucleic Acids Research 18:299-304 (1990).
Hampel and Tritz, "RNA Catalytic Properties of the Minimum (-)sTRSV Sequence," Biochemistry 28:4929-4933 (1989).
Haseloff and Gerlach, "Simple RNA Enzymes with New and Highly Specific Endoribonuclease Activities," Nature 334:585-59 (1988).
Hertel et al., "Numbering System for the Hammerhead," Nucleic Acids Res. 20:3252 (1992).
Jeffries and Symons, "a Catalytic 13-mer Ribozyme," Nucleic Acids Research 17:1371-1377 (1989).
Kim and Cech, "Three-dimensional model of the active site of the self-splicing rRNA precursor of Tetrahymena," Proc. Natl. Acad. Sci. USA 84:8788 (1987).
Mamone et al., "Design of Mannerhead Ribozymes Targeted in Sequences in HIV, HSV, and the RAT ANF GENE," Abstract of Keystone, CO (May 27, 1992).
Ng et al., "Isoguanosine Substitution of Conserved Adenosines in the Hammerhead Ribozyme," Biochemistry 33:12119-1216 (1994).
Odai et al., "The role of a conserved guanosine residue in the hammerhead-type RNA enzyme," FEBS 267:150-152 (1990).
Pavco et al., "Regulation of Self-Splicing Reactions by Antisense RNA," Abstract of Keytstone, CO (May 27, 1992).
Perreault et al., "Mixed Deoxyribo-and Ribo-Oligonucleotides with Catalytic Activity," Nature 344:565-567 (1990).
Perrotta and Been, "Cleavage of Oligoribonucleotides by a Ribozyme Derived from the Hepatitis .delta. Virus RNA Sequence," Biochemistry 31:16-21 (1992).
Pieken et al., "Kinetic Characterization of Ribonuclease-Resistant 2'-Modified Hammerhead Ribozymes," Science 253:314-317 (1991).
Pyle et al., "Ribozyme Recognition of RNA by Tertiary Interactions with Specific Ribose 2'-H Groups," Nature 350:628-631 (1991).
Rossi et al., "Ribozymes as Anti-HIV-1 Therapeutic Agents: Principles, Applications, and Problems," Aids Research and Human Retroviruses 8:183-189 (1992).
Saville and Collins, "A Site-Specific Self-Cleavage Reaction Performed by a Novel RNA In Neurospora Mitochondria," Cell 61:685-696 (1990).
Saville and Collins, "RNA-Mediated Ligation of Self-Cleavage Products of a Neurospora Mitochondrial Plasmid Transcript," Proc. Natl. Acad. Sci. USA 88:8826-8830 (1991).
Scaringe et al., "Chemical synthesis of biologically active oligoribonucleotides using .beta.-cyanoethyl protected ribonucleoside phosphoramidites," Nucl Acids Res. 18:5433-5441 (1990).
Seela et al., "122.7-Deazaadenosine: Oligoribonucleotides Building Block Synthesis and Autocatalytic Hydrolysis of Base-Modified Hammerhead Ribozymes," Helvetica Chimica Acta 76:1809-1820 (1993).
Slim and Gait, "Configuratoinally defined phosphorothioate-containing oligoribonucleotides in the study of the mechanism of cleavage of hammerhead ribozymes," Nucl Acids Res. 19:1183 (1991).
Tuschl et al., "Importance of Exocylic Base Functional Groups of Central Core Guanosines for Hammerhead Ribozyme Activity," Biochemistry 32:11658-11668 (1993).
Uhlenbeck, "A Small Catalytic Oligoribonucleotide," Nature 328:596-600 (1987).
Uhlmann and Peyman, "Antisense Oligonucleotides: A New Therapeutic Principle," Chemical Reviews 90:543-584 (1990).
Usman and Cedergren, "Exploiting the chemical synthesis of RNA," TIBS 17:334-339 (1992).
Usman et al.,"Automated Chemical Synthesis of Long Oligoribonucleotides Using 2'-O-Silylated Ribonucleoside 3'-O-Phosphoramidites on a Controlled-Pore Glass Support: Synthesis of a 43-Nucleotide Sequence Similar to the 3'-Half Molecule of an Escherichia coli Formylmethoionine tRNA,"J. Am. Chem. Soc. 109:7845-7854 (1987).
Vorbruggen et al., "Nucleoside Synthesis with Trimethylsilyl Triflate and Perchlorate as Catalysts," Chem. Ber. 114:1234-1255 (1981).
Weerasinghe et al., "Resistance to Human Immunodeficiency Virus Type 1 (HIV-1) Infection in Human CD4.sup.+ Lymphocyte-Derived Cell Lines Conferred by Using Retroviral Vectors Expressing an HIV-1 RNA-Specific Ribozyme," Journal of Virology 65:5531-5534 (1994).
Dropulic et al., "Functional Characterization of a U5 Ribozyme: Intracellular Suppression of Human Immunodeficiency Virus Type I Expression," Journal of Virology 66:1432-1441 (1992).
He et al., "In Vitro cleavage of HPV 16 E6 and E7 mRNA by designed ribozymes," Chemical Abstracts vol. 120, No. 1 at abstract No. 3342 (1994).
He et al., "In Vitro cleavage of HPV 16 E6 and E7 mRNA by designed ribozymes," FEBS Letters 322:21-24 (1993).
Lu et al., "Ribozyme-mediated in vitro cleavage of transcripts arising from the major transforming genes of human papillomavirus type 16," Cancer Gene Therapy 1(4):267-277 (1994).
Rossi and Sarver, "RNA enzymes (ribozymes) as antiviral therapeutic agents," TIBTECH 8:179-183 (1990).
Rossi et al., "Ribozymes as potential therapeutic agents," Proc. Annu. Meeting Am. Assoc. Cancer Res. 34:592-593 (1993).
Rossi et al., "Ribozymes As Therapies For AIDS," Annals of the New York Academy of Sciences 606:184-200 (1990).
von Weizsacker et al., "Cleavage of Hepatitis B Virus RNA by Three Ribozymes Transcribd From a Single DNA Template," Biochemical and Biophysical Research Communications 189:743-748 (1992).
Wong-Staal, "Molecular targets for interference with viral replication," International Conference on AIDS 9(1):10 (1993).
Beigelman Leonid
Karpeisky Alex
McSwiggen James
Usman Nassim
Elliott George C.
Garry Sean M.
Ribozyme Pharmaceuticals Inc.
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