Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1996-02-09
1998-03-24
Grimes, Eric
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
435839, C12N 910
Patent
active
057311838
ABSTRACT:
The present invention relates to (1) a transglutaminase (hereinafter referred to as TG) isolated from a Bacilli such as those of Bacillus subtilis, (2) a fraction having transglutaminase activity, and (3) a method for producing a protein, a non-proteinaceous amino acid polymer, a peptide or derivatives thereof having a crosslinked structure, by crosslinking the glutamine and lysine residues in the same with the TG or the fraction having TG activity to thereby form intermolecular or intramolecular, crosslinked .epsilon.-(.gamma.-Glu)-Lys bonds. The present invention also relates to (4) a DNA coding for a TG derived from a Bacilli such as Bacillus subtilis, (5) a vector comprising said DNA coding for the TG, (6) a cell transformed with the vector, and (7) a method for producing a Bacillus-derived transglutaminase by incubating the transformant. The crosslinked polymers produced using the Bacillus-derived TG of the present invention can be used in foods such as tofu (soybean curd), pudding, yogurt, cheese, ground fish meat, boiled fish paste, sausage and other fish and meat products and also in cosmetics, etc.
REFERENCES:
patent: 5156956 (1992-10-01), Motoki et al.
The Journal of Biological Chemistry, vol. 246, No.4, pp. 1093-1098, Feb. 25, 1971, John M. Connellan, et al. "Structural Properties Of Guinea Pig Liver Transglutaminase".
Journal of Bacteriology, vol. 174, No. 8, pp. 2599-2605, Apr. 1992, Janet D. Klein, et al., "Purification and Partial Characterization Of Transglutaminase From Physarum Polycephalum".
The FASEB Journal, vol. 4, No. 7 p. A2321, 1990, "Protein Processing And Turnover (3627-3632)".
Brock et al., Biology of Microorganisms, Prentice-Hall, Inc., Englewood Cliffs, NJ, pp. 725-727, 1984.
Eto Yuzuru
Hashiguchi Ken-ichi
Kobayashi Katsunori
Miwa Kiyoshi
Suzuki Shunichi
Ajinomoto Co. Inc.
Grimes Eric
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