Assays and kits incorporating nucleic acid probes containing imp

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 911, 935 77, 935 78, C12Q 168, C12P 1934

Patent

active

053127285

ABSTRACT:
A probe for the detection of a nucleic acid target sequence containing a molecular switch comprising three essential elements: a probe sequence of 20-60 nucleotides surrounded by switch sequences of 10-40 nucleotides which are complementary to each other, wherein the state of the switch is useful for selectively generating a detectable signal if the probe is hybridized to a target; also, assays and kits utilizing such probes.

REFERENCES:
patent: 4551433 (1985-11-01), DeBoer
patent: 4683202 (1987-07-01), Mullis
patent: 4710464 (1987-12-01), Belagaje et al.
patent: 4725537 (1988-02-01), Fritsch et al.
E. Engelke et al., "Direct sequencing of enzymatically amplified human geic DNA", Proc. Natl. Acad. Sci. USA 85:544-548 (1988).
B. Kalisch et al., "Covalently linked sequencing primer linkers (splinkers) for sequencing analysis of restriction fragments", Gene 44:263-270 (1986).
S. Krawetz et al., "Covalently linked complementary oligodeoxynucleotides (splinkers) as tools for molecular biology," Nucleic Acids research 14:7131 (1986).
Kramer, et al., "Replicatable RNA reporters", Nature 339:401-402 (1989).
Nishihara, Tohru, et al., "Localization of the Q Replicase Recognition Site in MDV-1 RNA", J. Biochem. 93:669-674.
Chu, et al., Synthesis of an amplifiable reporter RNA for bioassays 14(14) Nuc. Acids Res. 5591-5603 (1986).
Szybalski, Universal research in endonucleases: designing novel cleavage specificities by combining adapter oligodeoxy nucleotide and enzyme moieties. 40 Gene 169-73 (1985).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Assays and kits incorporating nucleic acid probes containing imp does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assays and kits incorporating nucleic acid probes containing imp, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assays and kits incorporating nucleic acid probes containing imp will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-876103

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.