Assay with reduced background

Details Assay with reduced background Assay with reduced background

2005-07-05

2005-07-05

Housel, James (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007100, C435S004000, C435S183000, C435S184000, C435S188500, C435S194000, C436S518000, C436S175000

Reexamination Certificate

active

06913896

ABSTRACT:
In as assay, an analyte is specifically associated with a reporter adenylate kinase, ADP is added and then formation of ATP is monitored. Prior to addition of ADP, adenylate kinase other than reporter adenylate kinase is removed. Assay apparatus comprises a solid phase on which is immobilised the analyte or an antibody specific for the analyte, a reporter composition comprising a thermostable adenylate kinase coupled to an antibody specific for the analyte, and ADP plus associated reagents for conversion of ADP into ATP by thermostable adenylate kinase.

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