Assay to detect the presence of live non-A, non-B hepatitis agen

Details Assay to detect the presence of live non-A, non-B hepatitis agen Assay to detect the presence of live non-A, non-B hepatitis agen

1988-12-06

1990-08-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 29, 435 32, 435236, 436820, C12Q 170, C12Q 102, C12Q 118

Patent

active

049524941

ABSTRACT:
An assay to detect the presence of live non-A, non-B hepatitis virus in vitro. Bone marrow cells are exposed to a body fluid or biological preparation to be tested and the cells are placed in suspension. When using bone marrow cells, growth factors are added to the bone marrow stem cells. Therefore, if the number of colonies growing in the mixture are less than that number present in the culture of cells exposed to a sample that has been determined to contain no live virus, live non-A, non-B hepatitis virus is present in the sample tested. The assay is particularly useful to determine the presence of live non-A, non-B, hepatitis virus in a vaccine.

REFERENCES:
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