Assay for the presence, in cultured cells, of functional ligand-

Details Assay for the presence, in cultured cells, of functional ligand- Assay for the presence, in cultured cells, of functional ligand-

1991-05-06

1993-05-04

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 20, 435 29, 436501, 436504, C12Q 168, C12Q 146, C12Q 102, G01N 33566

Patent

active

052081451

ABSTRACT:
There is provided a simple method to identify cation channels expressed in cultured cells and to screen chemical agents for their use as agonists or antagonists to such channels. In addition, the invention assay method also provides rapid means to identify cultured cell lines which express functional ion channels. The invention assay method can readily be adapted for the low cost, automated screening of potential drug materials.

REFERENCES:
Honchar et al., (1983) Science 220:323-325.
Aoshima (1983) J. Biochem. 94:1739-1751.
Neher et al., (1992) Scientific American Mar. 1992.

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