Assay for aminoglycosides

Details Assay for aminoglycosides Assay for aminoglycosides

1980-08-25

1982-05-18

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435193, 435 32, C12Q 148

Patent

active

043306217

ABSTRACT:
A method for assaying a biological fluid, e.g. blood, for aminoglycoside content which includes rendering X-SH groups in the fluid insoluble and removing the insolubilized groups, adding acetyl-coenzyme A and a thiol detecting reagent to the solution and measuring its optical density, adding an enzyme characterized by high specific activity against aminoglycosides, measuring the optical density of the resulting solution and determining the difference in the optical densities.

REFERENCES:
patent: 4073694 (1978-02-01), Buda et al.

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