Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-11-01
1998-10-13
Wax, Robert A.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 237, C07H 2104
Patent
active
058213503
ABSTRACT:
Disclosed are a cloned cDNA and a cloned genomic DNA, both encoding a .beta.-galactosidase polypeptide from Aspergillus niger. Also disclosed are .beta.-galactosidase expression vectors, transformed host cells that overexpress recombinant A. niger .beta.-galactosidase, and A. niger host cell strains with disrupted endogenous .beta.-galactosidase genes. Also disclosed are transgenic non-human mammals that express A. niger .beta.-galactosidase.
REFERENCES:
patent: 3629073 (1971-12-01), Cayle
patent: 3919049 (1975-11-01), Kiuchi et al.
Yamamoto, Yoshimi et al., DNA and Cell Biology, Isolation, Characterization, and Mapping of a Human Acid .beta.-Galactosidase CDNA, vol. 9, No. 2, pp. 119-127 (1990).
Greenberg, N.A. et al., J. Food Sci., "Rapid Purification of .beta.-Galactosidase (Aspergillus niger) from a Commercial Preparation", vol. 46, No. 3, pp. 684-687 (1981).
den Herder, I.F. et al. (1992) "Cloning and expression of a member of the Aspergillus niger gene family encoding alpha-galactosidase" Mol. Gen. Genet. 233:404-410.
Corazza et al., "62 -Galactosidase from Aspergillus niger in adult lactose malabsorption: a double-blind crossover study," Aliment. Pharmacol. Therap. 6:61-66 (1992).
Gouka et al., "A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori," Curr. Genet. 27:536-540 (1995).
Jacobson et al., "Three-dimensional structure of .beta.-galactosidase from E. coli," Nature 369:761-765 (1994).
Jeenes et al., "A truncated glucoamylase gene fusion for heterologous protein secretion from Aspergillus niger," FEMS Microbiology Letters 107:267-272 (1993).
Kumar et al., "Saccharomyces Cerevisiae Cells Secreting an Aspergillus Niger .beta.-Galactosidase Grow on Whey Permeate," Bio/Technology 10:82-85 (1992).
Nevalainen, "Induction, Isolation, and Characterization of Aspergillus niger Mutant Strains Producing Elevated Levels of .beta.-Galactosidase," Applied and Environmental Microbiology 41:593-596 (1981).
Oakley et al., "Cloning, mapping and molecular analysis of the pyrG (orotidine 5'-phosphate decarboxylase) gene of Aspergillus nidulans," Gene 61:385-399 (1987).
Paige et al., "Lactose hydrolyzed milk," Am. J. Clin. Nutrition 28:818-822 (1975).
Poch et al., "Sequence of the Kluyveromyces lactis .beta.-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis," Gene 118:55-63 (1992).
Ramakrishnan et al., "Fermentation of Lactose by Yeast Cells Secreting Recombinant Fungal Lactase," Applied and Environmental Microbiology 59:4230-4235 (1993).
Rings et al., "Lactase: Origin, Gene Expression, Localization, and Function," Nutrition Research 14:775-797 (1994).
Rosado et al., "The Effect of the Digestion Products of Lactose (Glucose and Galactose) on Its Intra-intestinal, In Vivo . . . Beta-D-Galactosidase," J. Am. College of Nutrition 5:281-290 (1986).
Solomons et al., "Effective in vivo hydrolysis of milk lactose by beta-galactosidases in the presence of solid goods.sup.1-3," Am J. of Clin. Nutrition 41:222-227 (1985).
Ward et al., "A System for Production of Commercial Quantities of Human Lactoferrin: A Broad Spectrum Natural Antibiotic," Bio/Technology 13:498-502 (1995).
Widmer et al., ".beta.-Galactosidase from Aspergillus niger," Eur. J. Biochem 100:559-567 (1979).
Delaquis Annick
Huang Yue
Karatzas Costas N.
Lazaris-Karatzas Anthoula
Lau Kawai
Nexia Biotechnologies Inc.
Wax Robert A.
LandOfFree
Aspergillus niger beta-galactosidase gene does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Aspergillus niger beta-galactosidase gene, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aspergillus niger beta-galactosidase gene will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-314202