Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-08-31
1999-08-17
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, C12Q 168
Patent
active
059392673
ABSTRACT:
Methods for determining the induction and assessing the course of programmed cell death (PCD) over time in living cells are provided. The methods of the present invention comprise the steps of maintaining a viable cell on a slide, staining the cell with annexin V to label any phosphatidylserine residues which may be present on the cell surface, visualizing the stained cell, removing the stain, sustaining the cell in culture, restaining the cell with annexin V to again label any phosphatidylserine residues present on the cell surface and visualizing the stained cell. The methods of the present invention are performed with cells maintained in a viable state, thereby allowing detection of the induction and assessment of the progression of PCD over time.
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Maiese Kenneth
Vincent Andrea M.
Ketter James
Wayne State University
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