Arginine deminase derived from mycoplasma arthritidis and polyme

Details Arginine deminase derived from mycoplasma arthritidis and polyme Arginine deminase derived from mycoplasma arthritidis and polyme

1997-01-31

1998-09-08

Hendricks, Keith D.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424 941, 435227, 536 231, 536 232, 536 237, A61K 3846, A61K 3843, C12N 978

Patent

active

058041834

ABSTRACT:
A purified arginine deiminase (ADI) obtained from Mycoplasma arthritidis having the amino acid sequence of SEQ ID NO:2 as well as an isolated nucleic acid molecule containing a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:1 are disclosed. Other aspects of the invention include an expression vector, a cloned gene for expressing the Mycoplasma arthritidis derived ADI, (recombinant) host cells useful in expressing the ADI of the present invention and substantially non-antigenic polymer conjugates containing the ADI of the present invention as well as methods of treating arginine deiminase susceptible conditions in mammals. The arginine deiminase-polymer conjugates have high levels of retained enzyme activity and relatively long circulating lives.

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