Apparatus for quantitative imaging of multiple fluorophores

Radiant energy – Luminophor irradiation

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2504591, G01N 2164

Patent

active

052947998

ABSTRACT:
A quantitative fluorometer for multiple fluorophores having a separate time-modulated beam of excitation light for each fluorescent target. Each beam is synchronized with a separate lock-in amplifier. The fluorophores are simultaneously excited and the combined fluorescent emission is resolved into components corresponding to each fluorophore. Confocal scanning means are used to excite and detect fluorescent emission from locations throughout a volume. The location specific output of each amplifier is stored in a computer which resolves the emission into the components corresponding to each fluorophore. The location specific data may be further processed or visually displayed.

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