Apparatus for measuring free and total sulfurous acid and method

Details Apparatus for measuring free and total sulfurous acid and method Apparatus for measuring free and total sulfurous acid and method

1991-10-04

1995-01-31

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 436 20, 436100, 436119, 436121, 436122, 436123, 426535, 426547, C12S 300, C12P 104, C12P 1100, C12M 100

Patent

active

053858301

ABSTRACT:
The present invention provides a method for measuring the amount of free sulfurous acid and bound sulfurous acid in a sample using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans of in which the sample is treated with acid and/or alkali to give free sulfurous acid alone in the sample. Then, the total amount of free sulfurous acid in the sample is measured by an oxidation reaction of free sulfurous acid to sulfuric acid using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans.

REFERENCES:
Cooper The Tools of Biochemistry John Wiley & Sons pp. 12, 13 1977.
Kim et al. J Food Science vol. 51, No. 5, 1986.
Nakamura, Appl Microbiol Biotech 31: 351-354 1989.
Silverman, et al. (1959) J. Bacteriology 77:642-647.
Mulchandani, et al. (1991) J. Biotechnology 16:93-102.

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