Apparatus for floating animal cells in a double-bag container

Details Apparatus for floating animal cells in a double-bag container Apparatus for floating animal cells in a double-bag container

1989-05-10

1991-10-15

Lacey, David L.

Chemistry: molecular biology and microbiology

Apparatus

Including condition or time responsive control means

435311, 435316, 604410, 604903, 383 37, 2062131, C12M 302

Patent

active

050574299

ABSTRACT:
The instant invention relates to an apparatus for floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.

REFERENCES:
patent: 2494866 (1950-01-01), Fressola
patent: 4183677 (1980-01-01), De Bruyne
patent: 4293643 (1981-10-01), Ohtake et al.
patent: 4661455 (1987-04-01), Hubbard
patent: 4829002 (1989-05-01), Pattillo et al.

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