Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1984-01-06
1986-03-04
McGraw, Vincent P.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
2504612, 356 39, 356 73, 356343, G01J 342, G01N 2164
Patent
active
045737967
ABSTRACT:
The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.
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Jett James H.
Martin John C.
Gaetjens Paul D.
Hightower Judson R.
Huffman Lee W.
McGraw Vincent P.
The United States of America as represented by the United States
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