Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1997-03-17
1998-10-06
Hantis, K.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
2504581, 2504591, G01J 330
Patent
active
058185828
ABSTRACT:
A phase fluorometer employs a baseband frequency generator to generate a sample baseband signal at a first frequency f.sub.o and a reference signal correlated to the sample baseband signal. A second signal generator generates a carrier signal having a second frequency f. The sample baseband signal and the carrier signal are combined to form an up-converted sample signal having a frequency f.+-.f.sub.o. A light source is modulated at the up-converted sample signal frequency f.+-.f.sub.o to illuminate a sample. A photo detector detects fluorescence emissions from a sample excited by the excitation light and converts the emission into a fluorescence signal. A down-converter combines the carrier signal and fluorescence signal to form a phase-shifted and down-converted sample signal. A processor digitally processes the phase-shifted and demodulated sample signal and the reference signal to determine the fluorescence lifetime.
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Cobane Sean
Fernandez Salvador M.
Gulgon Ernest F.
Levy Ralph
St. Louis Ernest
Ciencia, Inc.
Hantis K.
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