Antimicrobial activity of gemfibrozil and related compounds...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

Reexamination Certificate

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C435S189000

Reexamination Certificate

active

06881553

ABSTRACT:
The present invention provides a method of selecting a compound which inhibits the enzymatic activity of enoyl reductase which comprises: (A) contacting enoyl reductase with the compound linked to an acyl carrier protein; (B) measuring the enzymatic activity of the enoyl reductase of step (A) compared with the enzymatic activity of enoyl reductase in the absence of the compound and selecting the compound which inhibits the enzymatic activity of enoyl reductase.

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Bergler et al., The enoyl—[acyl carrier protein] reductase (FabI) ofEscherichia coli,which catalyzes a key regulatory step in fatty acid biosynthesis, accepts NADH and NADPH as cofactors and is inhibited by palmitoyl-CoA. (1996) Eur. J. Biochem. 242:689-694.
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