Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-05-17
2000-08-15
Woodward, Michael P.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 435 693, 536 2372, A61K 3929, A61K 3900
Patent
active
06103519&
ABSTRACT:
A composite particle comprising at least two polypeptides corresponding to all or part of a protein having the biological activity of one of the hepatitis B surface antigens, wherein the particle presents at least two antigenic determinants provided by the S-protein, preS2-protein or preS1-protein, said particle optionally further comprising host specific lipids. The invention also provides a modified hepatitis B surface antigen L protein which may be used alone or incorporated into such composite particles.
A particular embodiment of the modified L protein (L*) comprises residues 12-52 followed by residues 133-145 followed by residues 175-400 of the L protein and a particular embodiment of a composite particle has the form (L*, S) where S is the S-protein of HBsAg.
Improved hepatitis B vaccines may be prepared following expression of the above immunogens, especially in yeasts such as S. cerevisiae and Hansenula polymorpha.
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Ito
Cabezon Teresa
Comberbach Martin
Harford Nigel
Hollenberg Cornelis P.
Jacobs Eric
Kerekes Zoltan
Kinzig Charles M.
SmithKline Biologicals, S.A.
Venetianer Stephen
Woodward Michael P.
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