Antibodies for apoptosis EI24 protein and methods of use

Details Antibodies for apoptosis EI24 protein and methods of use Antibodies for apoptosis EI24 protein and methods of use

2006-07-18

2006-07-18

Shukla, Ram R. (Department: 1633)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C530S387100, C530S387200, C530S387300, C530S388100, C530S389100, C530S391100, C530S391300, C435S188000

Reexamination Certificate

active

07078175

ABSTRACT:
Disclosed is the isolation and characterization of EI24, a gene whose 2.4 kb mRNA is induced following etoposide treatment. Induction of EI24 mRNA by etoposide required expression of wild-type p53. Overexpression of functional p53 was sufficient to induce expression of the EI24 mRNA. The EI24 mRNA was also induced in a p53-dependent manner by ionizing irradiation of primary murine thymocytes. The invention is thus directed to an isolated EI24 protein, nucleotide sequences coding for and regulating expression of the protein, antibodies directed against the protein, and recombinant vectors and host cells containing the genetic sequences coding for and regulating the expression of the protein sequence. Antibodies can be used to detect EI24 in biological specimens, including, for example, human tissue samples.

REFERENCES:
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Friedlander et al., “A Mutant p53 that Discriminates Between p53-Responsive Genes Cannot Induce Apoptosis”,Molecular and Cellular Biology, vol. 16(9), pp. 4961-4971. Sep. 1996.
Ludwig et al., “Differential Activation of Target Cellular Promoters by p53 Mutants with Impaired Apoptotic Function”,Molecular and Cellular Biology, vol. 16(9), pp. 4952-4960. Sep. 1996.
Verma et al., “Gene Therapy—Promises, Problems and Prospects,”Nature, vol. 389, pp. 239-242. Sep. 1997.
Anderson, W.F., “Human Gene Therapy”,Nature, vol. 392, pp. 25-30. Apr. 1998.
Gu et al., “EI24, a p53 Response Gene Involved in Growth Suppression and Apoptosis”,Molecular and Cellular Biology, vol. 20(1), pp. 233-241. Jan. 2000.

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