Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2000-02-07
2001-07-24
Leary, Louise N. (Department: 1623)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S975000
Reexamination Certificate
active
06265182
ABSTRACT:
This invention related to a method and product that enable the determination of the susceptibility of bacteria to antibacterials in a short period of time. The subject matter of this invention may be applied to various fields of microbiology other than methods used for diagnosis of diseases.
PRIOR ART
In the prior art, disk diffusion and broth or agar dilution methods are the most commonly used methods to determine the susceptibility of bacteria to antibiotics. These methods require overnight incubation. In recent years, various methods to determine quickly the growth of bacteria at a certain amount of antibacterial, have been developed. These systems are usually computerized and require an instrument to detect bacterial growth. Examples are the following:
Turbidometric methods which determine the turbidity of the media that occurs due to bacterial growth.
Nephelometric methods that determine the growth of bacteria by detecting the change in the light scattering of a light beam directed to the medium.
Colorimetric methods that determine the change in color of the media due to bacterial growth that either produce different colored products by using the substrates provided in the media or by pH indicators that change color due to pH change.
Radiometric methods that measure the radioactive carbon dioxide produced from a radioactive carbon source in the medium that is used during bacterial growth.
Fluorometric methods that measure the light emitted from fluorescent substances produced during bacterial growth.
Methods that measure the change in impedance due to the change in electric current conductivity of the medium during bacterial growth.
For the application of these methods, at least one instrument to measure light, color, electric conductivity or radioactivity is required. However, the kit which is the subject matter of this invention does not require an instrument, and the results can be directly obtained by visual observation. However, it can be easily adapted to automation by a suitable optical reader.
BRIEF DESCRIPTION OF THE INVENTION
The aim of this invention is to provide a method that will decrease the time required to perform antibacterial susceptibility testing to 4 to 6 hours which usually takes 18 to 24 hours by classical testing systems.
Another aim of this invention is to enable performing antibacterial susceptibility testing easily without requiring preparatory work or material.
Yet another aim of this invention is to produce an antibacterial susceptibility test kit that does not require instrumentation that can be performed in a basic laboratory and that can be easily adapted to automation if desired.
REFERENCES:
patent: 4820428 (1989-04-01), McLennan et al.
patent: 94/16079 A1 (1994-07-01), None
Cruz, F.O., Database WPI on EPOQUE, week 9109, London:Derwent Publications, Ltd., AN 91-058363, Class D16, BR 8903571 A, abstract.
Leary Louise N.
Nixon & Vanderhye
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