Anti-HIV ribozymes

Details Anti-HIV ribozymes Anti-HIV ribozymes

1996-05-29

1997-12-09

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9131, 4351721, 4351723, 4353201, 435366, 4353723, 536 231, 536 232, 536 245, C12Q 168, C12N 1585

Patent

active

056959389

ABSTRACT:
Ribozymes targeted against two portions of the HIV-1 genome were designed to cleave HIV RNA in the tat gene (TAT) or in a common exon for tat and rev (TR). The ribozymes were cloned into the LN (LTR-neomycin) retroviral vector plasmids and expressed as part of vital LTR-driven transcripts. The vectors were packaged as amphitropic virions and used to transduce human T-lymphocytes. Expression of the vector transcripts containing the ribozyme sequences were readily detected by Northern blot analysis of the transduced T cells. The T-lymphocytes expressing the anti-HIV-1 ribozymes showed resistance to HIV-1 replication.

REFERENCES:
Guatell: et al. J. Cell. Biochem. Supple 16E 1992:79 #Q521.
Johnston et al. 260: 1286-1293 (1993), Science.
Barinaga Science 262: 1512-1514 (1993).
Sarver et al. Science 246:1222-1225 (1990).
Miller et al. Biotechniques 7:980-990 (1989).
Ratner et al. Nature 313:277 (1985).
Zhon et al. Gene 149:33-39 (1994).
Rossi et al. Derwent Biotechnology Asbstr. No: 95-00442.
Wong et al. Blood V. 84, No. 10 Nov. 15, 1994 pp. 743a, #2957.

Affiliated with

Also associated with

Rating

Anti-HIV ribozymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Anti-HIV ribozymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Anti-HIV ribozymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1606715