Analytical assay method

Details Analytical assay method Analytical assay method

1989-07-19

1992-11-24

Rosen, Sam

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

435 71, 435970, 436 8, 436517, 436518, 436536, 436800, G01N 33533

Patent

active

051660792

ABSTRACT:
A method for determining the amount of an analyte in a sample fluid utilizes a multilayer assay element which comprises at least one reagent layer and a light-blocking layer. The assay method includes the steps of optically reading a signal producing species, e.g. a fluorescent label, a first time before the sample fluid is applied to the assay element and a second time, at the same wavelength and in the same location within the assay element, after the sample fluid has been applied to the assay element and the sample analyte has interacted with the reagent(s) present in the assay element. The ratio of the two signals is taken and compared with that for known amounts of the analyte to determine the amount of analyte in the sample fluid.

REFERENCES:
patent: 4457893 (1984-07-01), Takehawa
patent: 4459358 (1984-07-01), Berke
patent: 4832488 (1989-05-01), Hirai et al.

Affiliated with

Also associated with

Rating

Analytical assay method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Analytical assay method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Analytical assay method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-921345