Analysis element and method for analyzing glycated hemoglobin co

Details Analysis element and method for analyzing glycated hemoglobin co Analysis element and method for analyzing glycated hemoglobin co

1995-10-17

1999-03-16

Hutzell, Paula K.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

Glycosylated hemoglobin

435 79, 435 791, 435 282, 435 28, 436 66, 436523, 422 8205, H01L 21302, H01L 21304, H01L 21306, H01L 2176

Patent

active

058829350

ABSTRACT:
An analysis element for analyzing both amounts of glycated hemoglobin and total hemoglobin in an aqueous liquid sample to determine glycated hemoglobin content ratio in the sample. The element comprises a substrate layer for receiving a reaction mixture after the completion of an immunological reaction between the glycated hemoglobin in the sample and an enzyme-labelled antibody against the glycated hemoglobin, and a reagent layer. The substrate layer contains a non-diffusible substrate which forms a diffusible material in the presence of the enzyme of the enzyme-labelled antibody, the activity of the enzyme being effected relative to the steric hindrance due to the immunological reaction. The reagent layer contains a reagent composition for reacting with the diffusible material to form a dye detectable colorimetrically in a wavelength range which is not effected by an absorption spectrum of the hemoglobin. The total hemoglobin retained in the substrate layer and the dye formed in the reagent layer are colorimetrically analyzed separately, and the glycated hemoglobin content ratio is calculated from respective values. An analysis method using this analysis element is also provided.

REFERENCES:
patent: 5093081 (1992-03-01), Sudo et al.
Database WPI--Section Ch, Week 9224--Derwent Publication & JP A 04 128 655 (Fuji Photo Film Co. Ltd.).

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