Amylases

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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Details

C435S069100, C435S183000, C435S195000, C435S200000, C435S203000, C435S204000, C435S205000, C435S252300, C435S320100, C536S023200, C536S023700

Reexamination Certificate

active

06916645

ABSTRACT:
Liquefying alkaline amylases, each having residual activity not less than 70% when treated at pH 10 and 45° C. for 30 minutes in the presence of 1 to 100 mM of EDTA or EGTA, are described. Also described are detergents comprising these amylases. In comparison with conventional amylases for detergents, the liquefying alkaline amylases of this invention have a high chelating-agent resisting performance.

REFERENCES:
patent: 0 670 367 (1995-09-01), None
patent: WO 9011352 (1990-10-01), None
patent: WO 9426881 (1994-11-01), None
patent: WO 9502639 (1995-01-01), None
patent: WO 9602633 (1996-02-01), None
Dong et al., Cloning, Sequencing, and Expression of the Gene Encoding Extracellular α-Amylase fromPyrococcus furiosusand Biochemical Characterization of the Recombinant Enzyme, Applied and Environmental Microbiology, Sep. 1997, vol. 63, pp. 3569-3576, XP-002201543.
Mischa Machius et al, Activation ofBacillus licheniformisα-amylase through a disorder order transition of the substrate-binding site mediated by a calcium-sodium-calcium metal triad, Jan. 12, 1998.
Edited by Jan H. Van Ee et al, Enzymes in Detergency, copyright 1997 by Marcel Dekker, Inc.
Edited by The Amylase Research Society of Japan, Handbook of Amylases and Related Enzymes, copyright 1988 by Pergamon Press.

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